Analysis of the Human {-Globin Gene Promoter in Transgenic Mice
نویسندگان
چکیده
{-Globin is the embryonic form of the O( chain of hemoglobin. Transgenic mice generated with {-globin constructs containing the {-globin gene, 557 bp of 5 flanking sequence, and 2-kb of 3 flanking sequence linked to the @globin locus control region hypersensitive site 2 (HS2) expressed human {-globin only in embryonic yolk sac erythroid tissue, and not in definitive erythroid tissue in the fetal liver or in adult peripheral blood. To determine what sequences in the 5 flanking region of the {-globin gene might be important for developmental specificity, a series of 5 deletion constructs of the {-globin gene were made and used to generate transgenic mice. The 5 ends of these
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